Lnk is overexpressed in MPNs. (A-B) Relative expression of total LNK mRNA was determined by real-time PCR using either the TaqMan method on purified platelets (A) or the SYB Green method on sorted CD34⁺ cells (B). Relative LNK mRNA levels are expressed in arbitrary units (A) or log2 (B) of ratios obtained using housekeeping genes for normalization. The horizontal line in the box marks the median and bars indicate the range of values. All P values were 2-sided with P ≤ .05 denoting statistical significance: *P = .0076; **P < .0001; ***P = .004. All patients were studied in duplicate. RT, reactive thrombocytosis; PV, polycythemia vera; PMF, primary myelofibrosis.