VPA/AZT treatment to an accumulation of CD8⁺ effector memory T lymphocytes. (A) Variation of CD3⁺, CD4⁺, and CD8⁺ cell number per cubed millimeter of blood was determined at days 0 and 7 of treatment. Cells were stained with CD4, CD8, and CD3 antibodies and analyzed by flow cytometry. The absolute numbers are derived from the absolute lymphocyte count previously determined by clinical automated systems. (B) Variation in the CD8⁺ subpopulations count was determined at days 0 and 7 of treatment. Cells were stained for CD3, CD8, CD95, and CD28. *Significant difference (P < .05; paired Student t test).