IL-4 impairs uptake of RdGnX-N meningitidis and increases antibacterial proinflammatory response in vivo. (A left) Flow cytometry profile of uptake of RdGnX-N.m. (Nm) by peritoneal cells. RdGnX-N meningitidis cells (108) were injected in the peritoneum of C57/BL6J mice. Animals were preinjected with IL-4, and the inflammatory infiltrate was collected by lavage. Mice preinjected with PBS were used as control. (Right) Scatter plot showing effect of IL-4 on RdGnX-N meningitidis uptake by MΦs. (B) Cytokine ELISA for TNF-α and IL-6 production in peritoneal lavage fluid. Ethanol-killed N meningitidis cells (2 × 106) were injected into the peritoneum of C57/BL6J mice, preinjected with IL-4. The inflammatory infiltrate was collected by lavage, and the supernatant was assayed for TNF-α and IL-6. Mice preinjected with PBS were used as controls. Each symbol represents 1 animal.
