Tie2-Egfl7 transgenic mice exhibit defects in vascular cell stratification at branch points of the dorsal aorta. Immunofluorescence staining of the dorsal aorta at E12.5. CD31⁺ cells are shown in green (Alexa Flour 488), and SMA⁺ cells are shown in red (Cy3). Images were captured on the TCS AOBS SP2 microscope (Leica). (A-B) Wild-type and transgenic dorsal aorta, respectively. Original magnification 20×/0.7NA, water objective. (C-D) High-magnification image of boxed areas in panels A and B. Original magnification 63×/1.2NA, water objective. (E) Summary of phenotypes observed in Egfl7 transgenic embryos.