American Society of Hematology

Figure 1

$Figure 1. Isolation and frequency of endosteal cell populations. (A) Representative FACS profile of ALCAM and Sca-1 expression in CD45−CD31−Ter119− cells in BM cells (top) and the bone-associated fraction (cells isolated from bone fragments using collagenase treatment, bottom). CD45−CD31−Ter119− cells were gated, and the expression of Sca-1 and ALCAM was analyzed. CD45−CD31−Ter119− cells were subdivided into 3 fractions: ALCAM+Sca-1−, ALCAM−Sca-1−, and ALCAM−Sca-1+ cells. (B) Frequency of ALCAM+Sca-1−, ALCAM−Sca-1−, and ALCAM−Sca-1+ populations in the CD45−CD31−Ter119− fraction in BM cells. Data represent means ± SD (*P < .01, n = 6). (C) Frequency of ALCAM+Sca-1−, ALCAM−Sca-1−, and ALCAM−Sca-1+ populations in the CD45−CD31−Ter119− fraction in bone-associated cells. Data represent means ± SD (*P < .01, n = 6).$

Isolation and frequency of endosteal cell populations. (A) Representative FACS profile of ALCAM and Sca-1 expression in CD45⁻CD31⁻Ter119⁻ cells in BM cells (top) and the bone-associated fraction (cells isolated from bone fragments using collagenase treatment, bottom). CD45⁻CD31⁻Ter119⁻ cells were gated, and the expression of Sca-1 and ALCAM was analyzed. CD45⁻CD31⁻Ter119⁻ cells were subdivided into 3 fractions: ALCAM⁺Sca-1⁻, ALCAM⁻Sca-1⁻, and ALCAM⁻Sca-1⁺ cells. (B) Frequency of ALCAM⁺Sca-1⁻, ALCAM⁻Sca-1⁻, and ALCAM⁻Sca-1⁺ populations in the CD45⁻CD31⁻Ter119⁻ fraction in BM cells. Data represent means ± SD (*P < .01, n = 6). (C) Frequency of ALCAM⁺Sca-1⁻, ALCAM⁻Sca-1⁻, and ALCAM⁻Sca-1⁺ populations in the CD45⁻CD31⁻Ter119⁻ fraction in bone-associated cells. Data represent means ± SD (*P < .01, n = 6).

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