Single-cell Q-PCR array. (A) Heat maps of the representative gene expression patterns of selected genes in ALCAM⁺Sca-1⁻ and ALCAM⁻Sca-1⁺ cells at the single-cell level. The horizontal axis in the heat map is a list of genes tested, including internal controls, and the vertical axis represents cell samples (16 independent cells in each fraction/experiment). The color in each cross point indicates delta-delta Ct (ddCt). Representative data from 2 (of 5) independent experiments are shown. (B) Expression levels of collagen type I alpha 1 (Col1a1), Runx2, alkaline phosphatase (Alp1), Ocn, Angpt1, Thpo, Cdh2, Cxcl12, Nanog, Pou5f1, Sox2, and Eng in each single cell.