Analysis of TAC4−/− and WT bone marrow. (A) Scatter gram representing the absolute number of bone marrow cells recovered from WT and TAC4−/− mice. (B) Four-color FACS analysis was performed on total bone marrow samples. The fold change of TAC4−/− B-cell populations compared with WT B-cell populations is shown based on percentage. (C) The fold change of the CD19+CD117+HSA+BP.1− “fraction B” pro-B-cell population compared with WT “fraction B” is shown based on the cell number of sorted cells. Means, P values, and levels of significance are indicated (n = 7-10). *P < .05. **P < .005. P values were calculated from percentages using paired 2-tailed t tests (GraphPad Prism software Version 3.0).