Analysis of IL-7 cultures derived from total bone marrow from WT and TAC4^−/− mice. (A) Four-color FACS analysis was performed on bone marrow cells grown in the presence of IL-7 for 4 days. The fold change of TAC4^−/− B-cell populations compared with WT B-cell populations is shown based on absolute cell numbers. Data are means, P values, and levels of significance (n = 10). *P < .05. P values were calculated from absolute numbers using paired 2-tailed t tests (GraphPad Prism software Version 3.0). (B) CFSE staining profiles are shown for WT and TAC4^−/− total bone marrow cultures over a time course of 4 days. The figure shows 1 representative experiment that has been performed multiple times with similar results.