UPR induction in P2 BLCL. (A) Immunoblot showing the expression level of GRp78 and GRp94 in control (C) and patient's BLCLs. HEK-293 cells, untreated (-) or treated for 6 hours with 2 different doses of Tunicamycin (TM) are shown as control of UPR induction. Tubulin was used as loading control. (B) Real-time PCR analysis of GRp78 and ERdJ4 mRNA expression, in C and mutant BLCLs, untreated or treated with TM. Expression values, relative to β-actin, are normalized to the expression observed in C. The experiment shown is representative of 2 independent experiments with triplicate samples, and data are given as means ± SD. (C) RT-PCR analysis of XBP1 mRNA, spliced (sXBP1) and unspliced (uXBP1). The experiment shown is representative of 2 independent experiments. Vertical lines have been inserted to indicate repositioned gel lanes.