Targeting of 3G9 to DCs in vivo. Wild-type and hDEC205 Tg mice were injected intraperitoneally with Alexa 647–labeled 3G9 or human IgG1 isotype (30 μg per mouse). Twelve hours later, lymphocyte lineage-negative (LIN⁻) splenic cell suspensions were stained with the indicated antibodies. In the histograms, blue is for wild-type (WT) and red for hDEC205 Tg mice (Tg).