CD14 secretion by monocytes is increased in the presence of CLL cells. CD14-sorted monocytes (1 × 105) were cultured in the absence or presence of the indicated numbers of CD19-sorted CLL cells in 1 mL of complete medium per well in 24-well plates for 2 days. Cell-culture supernatants were analyzed by ELISA for the presence of soluble CD14. Mean values and standard deviations of duplicates of one representative example of 3 independently performed experiments are shown.