Mouse platelets do not express TF. (A) Diagram showing the position of 2 sets of primers used to detect TF pre-mRNA and mRNA. The expected size of PCR products for primer sets A and B are shown. (B) PCR analysis of TF was performed on either mouse genomic DNA or cDNA from LPS stimulated RAW 264.7 macrophages, LPS stimulated mouse peripheral blood leukocytes, or LPS stimulated mouse platelets. Lane 10 contains platelet cDNA amplified with primers for integrin α_IIb. This gel is representative of 11 independent experiments. (C) Plasma TAT levels were analyzed in TF^flox/flox (n = 13; mean ± SEM 18.9 ± 2.1 ng/mL) and TF^flox/flox,PF4^Cre (n = 13; mean ± SEM 17.5 ± 2.2 ng/mL) mice 8 hours after LPS injection.