Primary WM cells present with higher level of miRNA-206, lower level of miRNA-9*, together with increased expression of HDACs and reduced expression of HATs, compared with healthy donors. (A) miRNA analysis has been performed on total RNA isolated from BM CD19⁺ WM cells, normal bone marrow (NBM)– and PBMC-derived CD19⁺ counterparts, and WM cell line (BCWM.1). Heatmap was generated after supervised hierarchical clustering analysis was performed with analysis of variance test. Differential expression of miRNA patterns is shown by the intensity of red (up-regulation) versus blue (down-regulation). (B) Purified cRNA (15 μg) isolated from primary CD19⁺ cells isolated from BM of 6 patients with WM and from CD19⁺ cells isolated from PBMCs of 2 healthy donors was hybridized to HG-U133Plus2.0 GeneChip (Affimetrix). Fold change is shown by the intensity of induction (red) or suppression (blue).