miRNA-9*–dependent HDAC inhibition exerts a proapoptotic effect on WM cells. (A) Percentage of cells undergoing apoptosis was studied by Apo2.7 staining and flow cytometry in WM and low-grade lymphoma IgM-secreting cell lines (untransfected, scramble probe–, pre-miRNA-9*–transfected were harvested 48 hours after transfection). All P ≤ .05. (B) BCWM.1 cells (untransfected, scramble probe–, and pre-miRNA-9*–transfected) were harvested at 12 hours after transfection. Whole-cell lysates were subjected to Western blot with the use of anti-PARP, –caspase-8, –caspase-9, and –actin antibodies. (C-D) BCWM.1 cells were cultured with LBH589 (0-60nM) for 16 hours. Whole-cell lysates were subjected to Western blot with the use of anti-p53, –BCL-XL, –Mcl-1, –c-myc, –β-actin, -PARP, –caspase-9, –caspase-8, –caspase-3, and –α-tubulin antibodies. (E) BCWM.1 cells were cultured with LBH589 for 48 hours at doses that range from 0 to 60nM, and the percentage of cells undergoing apoptosis was studied by Apo2.7 staining by flow cytometry. All P ≤ .05.