miRNA-9*–dependent HDAC inhibition and modulation of autophagy in WM cells. (A) BCWM.1 cells (untransfected, scramble probe–,and pre-miRNA-9*–transfected) were harvested at 24 hours after transfection. Whole-cell lysates were subjected to Western blot with the use of anti-Rab7, -LC3, and -actin antibodies. (B) BCWM.1 cells were cultured with LBH589 (40nM) for 0 to 24 hours. Whole-cell lysates were subjected to Western blotting with the use of anti-Rab7 and -LC3B antibodies. (C-D) BCWM.1 cells were cultured in the presence or absence of LBH589 (40nM) for 16 hours. Immunocytochemical analysis was assessed with the use of anti-Rab7 (C) or -LC3B (D) antibodies. DAPI indicates 4′-6′-diamidine-2-phenylindole.