IL-23 activity on pediatric B-ALL cell proliferation and apoptosis. (A) Flow cytometric analysis of proliferating cells in primary B-ALL cells cultured 24 or 48 hours with or without IL-23. Left panel: whisker lines represent highest and lowest values, horizontal lines represent median values. One representative staining is shown in the right panel. Open profile: Ki67 staining in B-ALL cells cultured in medium alone (med); dashed profile: Ki67 staining in B-ALL cells treated with IL-23; dark profile: isotype-matched mAb staining in B-ALL cells treated with IL-23. (B) Flow cytometric analysis of apoptotic (annexin V⁺/PI⁺) cells in primary B-ALL cells cultured 24 or 48 hours with or without IL-23. Results represent the mean percentage of annexin V/PI–positive cells ± SD from 5 samples. One representative experiment is shown in the right panel. (C) MiRNA expression profile of 6 pooled primary B-ALL samples cultured with or without IL-23. Histograms represent fold differences of individual miRNA (miR) expression between B-ALL cells cultured with and without IL-23.