Induction of BM failure with T-cell expansion in a mouse model. CByB6F1 mice (n = 5) were irradiated (5 Gy TBI) and injected with 5 × 10⁶ B6 LN cells and then bled and killed 10 days later along with untreated control mice (irradiated non–LN-injected, TBI-only, n = 5; non-irradiated non–LN-injected, untreated, n = 5). CBCs were performed using a Hemavet analyzer. BM cells were stained with anti-CD4 and anti-CD8 antibodies and examined by flow cytometry. Experiments are represented as mean ± SD. P less than .05 is considered statistically significant. (A) Representative flow cytometric analysis of IL-17 expressions in CD4⁺ (top panel) and CD8⁺ (bottom panel) T-cell subsets (Th17 cells) in B6LN, TBI-only, and untreated mice. The mean value of each group is indicated. (B) Absolute numbers of CD4⁺ (top panel) and CD8⁺ (bottom panel) T-cell subsets (Th17 cells) in B6 LN, TBI-only, and untreated mice. Experiments are represented as mean ± SD. (C) Representative flow cytometric analysis of IL-17 and IFN-γ coexpression in CD4⁺ (top panel) and CD8⁺ (bottom panel) T-cell subsets (Th17 cells) in B6 LN mice. The mean value of each group is indicated.