5azaD/SAHA treatment reduces the number of assayable PMF HPCs. (A) Total number of colonies assayed from the culture of PMF CD34⁺ cells (1 × 10⁵) in the presence of cytokines alone or cytokines plus SAHA for 6 days (n = 6). (B) Total number of colonies assayed from the culture of PMF CD34⁺ cells (1 × 10⁵) in the presence of cytokines alone or cytokines plus 5azaD/SAHA for 8 days (n = 15). *P < .05. (C) The degree of the expansion of progenitor cells after ex vivo culture was determined by dividing the number of colonies assayed from cultures performed in the presence of cytokines alone or cytokines plus 5azaD/SAHA by that assayed from input primary CD34⁺ cells (n = 15). *P < .05.