Figure 4
5azaD/SAHA treatment does not reduce the number of HPCs assayed from CB. CB CD34+ cells (1 × 105; n = 6) were treated with 5azaD/SAHA for 8 days in the same way as PMF CD34+ cells. (A) The absolute number of CD34+ cells in the culture of CB CD34+ cells (1 × 105) treated with cytokines alone or cytokines plus 5azaD/SAHA. (B) Total number of colonies assayed from the culture of CB CD34+ cells (1 × 105) in the presence of cytokines alone or cytokines plus 5azaD/SAHA. (C-D) The percentage and total number of CD34+ cells which expressed CXCR4 in CB CD34+ cells treated with cytokines alone or cytokines plus 5azaD/SAHA.
