CNA profiles and patterns of SHMs from 4 representative cases. (A-D) CNA profiles from cases 34, 5, 26, and 28, respectively. The chromosomes 1 to 22 and X are aligned along the x-axis from 1p36 to Xq28. Y-axis; smoothed log2 ratios, k = 29. Green, red, and blue lines represent the CNA profiles in biopsy 1, 2, and 3 from each patient, respectively. (E-H) Patterns of somatic hypermutations (SHMs) in samples from cases 34, 5, 26, and 28, respectively. Green, red, and blue dots represent biopsy 1, 2, and 3, respectively. The position of the dots along the x-axis represent the time point at which the biopsies were obtained relative to primary diagnosis (t = 0). Y-axis; number of SHMs. (A) Case 34. Biopsy 1 = FL grade 1 (t = 0). Biopsy 2 = FL grade 2 (t = 5.7). Biopsy 3 = FL grade 3a (t = 6.8). Gain of 18 is present in all 3 biopsies. Losses on 5q and 6q in biopsy 2 are absent in biopsy 3, whereas several new CNAs also appear in biopsy 3. (B) Case 5. Biopsy 1 = FL grade 1 (t = 0). Biopsy 2 = DLBCL (t = 1.2). Biopsy 3 = FL grade 2 (t = 2.4). Gain of 11q is present in all 3 biopsies. Losses of 15 and 17p in biopsy 1 are absent in biopsy 2. Losses of 2p and 13 and gains of 2p, 7q, and 16 in biopsy 2 are absent in biopsy 3. (C) Case 26. Biopsy 1 = FL grade 2 (t = 9). Biopsy 2 = FL grade 3a with marginal zone differentiation (t = 9.4). Loss of 13 appears in biopsy 2. (D) Case 28. Biopsy 1 = FL grade 1 (t = 0). Biopsy 2 = FL grade 1, (t = 0. 1). Gains of chromosome 3 and 9 in biopsy 2 are barely seen in biopsy 1. (E). Case 34. Sixteen mutations are common for biopsies 1 and 2, whereas 3 and 10 additional mutations, respectively, are unique. (F) Case 5. Biopsies 1 and 2 have 21 mutations in common in addition to 1 unique mutation each. Biopsy 3 has 14 mutations in common with the 2 preceding samples in addition to 13 unique mutations. (G) Case 26. Biopsies 1 and 2 have 24 mutations in common and 1 unique mutation each. (H) Case 28. Biopsies 1 and 2 have 33 mutations in common and 3 unique mutations each.
