Increased platelet accumulation in G_i2α(+/G184S) mice after laser injury in cremaster muscle arterioles. (A) Thrombus formation in G_i2α(+/G184S) mice and matched controls (+/+). Platelets labeled with fluorescently conjugated anti-CD41 Fab fragments are shown in green. Arteriole walls are outlined in red with the direction of blood flow indicated with an arrow. Video captures at selected time points are shown. Videos showing the entire period of observation are included in the online supplement. (B) Median fluorescence intensity versus elapsed time after laser injury. The dataset included 38 injuries observed in 5 G_i2α(+/G184S) mice and 33 injuries in 4 matched controls G_i2α(+/+).