MLN4924 is a potent inhibitor of NAE and induces distinct cell-cycle profiles analyzed by flow cytometry in ABC- and GCB-DLBCL cell lines. OCI-Ly10, OCI-Ly3, OCI-Ly19, and OCI-Ly7 cells were treated with MLN4924 for 24 hours. (A) Inhibition of NAE was assessed by immunoblot analysis of Ubc12-NEDD8 thioester and cullin-NEDD8 levels, respectively. The nonspecific band serves as the loading control. (B) DNA profiles were analyzed by propidium iodide staining and flow cytometry.