American Society of Hematology

Figure 2

$Figure 2. MLN4924 induces apoptosis through inhibition of NF-κB signaling in ABC-DLBCL and induction of DNA rereplication in GCB-DLBCL. OCI-Ly10 (A) and OCI-Ly19 (B) cells were treated with EC50, EC90, and 1μM concentrations of MLN4924 for 1, 2, 4, 8, 16, and 24 hours. Cell lysates were immunoblotted for NEDD8-cullin, phosphorylated IκBα (Ser32), Cdt-1, phosphorylated Chk1 (Ser317), phosphorylated H2AX (SER139, cleaved (Clvd) caspase 3, and tubulin. (C) OCI-Ly10 and OCI-Ly19 cells were treated with increasing concentrations of MLN4924 for 4 hours. The nuclear fraction was prepared and subjected to enzyme-linked immunosorbent assay-based quantitation of p65 levels. (D) OCI-Ly10 and OCI-Ly19 cells were treated with EC90 concentrations of MLN4924 for 1, 3, 6, 12, and 24 hours, and quantitative RT-PCR was performed to measure levels of NF-κB target gene transcripts.$

MLN4924 induces apoptosis through inhibition of NF-κB signaling in ABC-DLBCL and induction of DNA rereplication in GCB-DLBCL. OCI-Ly10 (A) and OCI-Ly19 (B) cells were treated with EC₅₀, EC₉₀, and 1μM concentrations of MLN4924 for 1, 2, 4, 8, 16, and 24 hours. Cell lysates were immunoblotted for NEDD8-cullin, phosphorylated IκBα (Ser32), Cdt-1, phosphorylated Chk1 (Ser317), phosphorylated H2AX (SER139, cleaved (Clvd) caspase 3, and tubulin. (C) OCI-Ly10 and OCI-Ly19 cells were treated with increasing concentrations of MLN4924 for 4 hours. The nuclear fraction was prepared and subjected to enzyme-linked immunosorbent assay-based quantitation of p65 levels. (D) OCI-Ly10 and OCI-Ly19 cells were treated with EC₉₀ concentrations of MLN4924 for 1, 3, 6, 12, and 24 hours, and quantitative RT-PCR was performed to measure levels of NF-κB target gene transcripts.

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