CAL-101 inhibits the PI3K/Akt and ERK pathway. (A) INA-6 cells were cultured with CAL-101 (0-1.0μM) or LY294002 (0-20μM) for 12 hours. Actin Ab was used as a loading control. (B) INA-6 and MM.1S cells were cultured with CAL-101 (0-5.0μM) for 6 hours. (C) LB and INA-6 cells were cultured with CAL-101 (1.0μM) for 0 to 6 hours. Whole-cell lysates were subjected to immunoblotting using AKT, P-AKT (Ser473 and Thr308), ERK1/2, P-ERK1/2, P-PDK1, and P-FKHRL Abs. α-Tubulin is used as a loading control.