CAL-101 inhibits paracrine MM cell growth and angiogenesis. (A) LB and INA-6 MM cells were cultured for 48 hours with control media and with 2.5, 5, and 10μM CAL-101 in the presence or absence of BMSCs. DNA synthesis was determined by [³H]-thymidine incorporation. Data are mean ± SD of triplicate cultures. (B) IL-6 in culture supernatants from BMSCs treated with CAL101 (0-2.5μM) was measured by ELISA. Error bars represent SD. (C) HUVECs were cultured with or without 1.0 or 10μM CAL-101 for 8 hours, and tube formation was assessed by microscopy. (D) HUVECs were plated on Matrigel-coated surfaces and allowed to form tubules for 8 hours in the presence or absence of CAL-101 (1.0 and 10μM). Endothelial cell tube formation was measured by microscopic analysis. *P < .005. (E) HUVECs were cultured with CAL-101 (0-200μM) for 48 hours, and viability was assessed by MTT assay. Data are mean ± SE of triplicate wells from a representative experiment.