Impaired T cell-stimulatory capacity of DCs resulting from mDia1 deficiency. (A-B) Alloreactive CD4⁺ T cells were purified from BALB/c mice and stimulated with or without B6 or mDia1^−/− BMDCs in 200 μL of cRPMI for 72 hours. The numbers of each cell subset are shown in the figure. ³H-Thymidine incorporation in triplicate wells as an indicator of T-cell proliferation (A) and levels of IFN-γ in the culture supernatant (B) were measured. (C-E) The expression levels of MHC class II (C), CD40 (D), and CD54 (ICAM-1) (E) of BMDCs at 24 hours and 48 hours. Data are mean ± SD and are representative of 3 independent experiments. *P < .05 vs corresponding B6 mice.