Further characterization of ILY-mediated hemolysis mouse model. (A-C) ThCD59^RBC erythrocyte susceptibility to lysis by different concentrations of NaCl (A), mellitin (B), or PLY (C) was similar to that of WT erythrocytes. Erythrocytes were incubated in serial concentrations of NaCl, mellitin, or PLY at 37°C for 30 minutes. Results represent mean ± SEM (n = 3, P > .05). (D) ILY-mediated hemolysis-associated sudden death. ILY₁₄₀FI induced 100% (12/12) mortality, but ILY₁₄₀SI (0/12) and ILY₇₀FI (0/8) did not cause death in any mouse. *P < .001 vs the mice with ILY₁₄₀FI. (E-F) Hematocrit- and plasma-free hemoglobin levels in the ThCD59^RBC and WT mice administered ILY injection. *P < .001 vs WT mice and #P < .01 vs the ThCD59^RBC mice administered ILY₇₀FI. Results represent mean ± SEM.