NO bioavailability and platelet activation in ILY-mediated hemolysis mice. (A) NO (measured as nitrite and nitrate) was reduced in ILY-injected ThCD59^RBC but not WT mice. *P < .05 vs WT. Values shown are mean ± SEM. (B-C) sP-selectin levels or platelet counts. *P < .05 vs WT with ILY₁₄₀FI and **P < .05 vs ThCD59^RBC with ILY₁₄₀FI. (D) ILY resulted in increased TAT in ThCD59^RBC but not in WT mice. Blood samples were collected 1 minute after ILY₁₄₀FI and 15 minutes after ILY₁₄₀SI and ILY₇₀FI from ThCD59^RBC and at 15 minutes after ILY₁₄₀FI from WT mice. Results represent mean values ± SEM. *P < .01 vs WT with ILY₁₄₀FI.