miR-155* and miR-155 have opposite effects on TLR7-mediated IFN-α/β and TNF-α production in human PDCs. Human PDCs were transfected with (A,C,E) mimic control (mimic-ctrl), miR-155 mimic (miR-155), or miR-155* mimic (miR-155*), (B,D,F) oligonucleotide control (oligo-ctrl), as-miR-155, or as-miR-155*, with Lipofectamine 2000. Eight hours after transfection, the PDCs were stimulated with 10 μg/mL R837 for 24 hours. IFN-α (A-B), IFNβ (C-D), and TNF-α (E-F) expression levels in the supernatants were measured by enzyme-linked immunosorbent assay (ELISA). The data are mean ± SD of triplicate wells from 1 of 3 experiments with similar results, analyzed with 2-tailed Student t test. *P < .05; **P < .01.