IRAKM and TAB2 negatively correlate with miR-155* and miR-155, respectively. (A-B) IRAKM and TAB2 had opposite effects on IFN-α and IFN-β production in human PDCs upon TLR7 stimulation. Purified human PDCs transfected with siRNA control, IRAKM siRNA, or TAB2 siRNA were stimulated with R837 for 24 hours. IFN-α (A) and IFN-β (B) in the supernatants were measured by ELISA. The data were from 1 of 3 experiments with similar results, analyzed with 2-tailed Student t test. *P < .05, **P < .01. (C-D) miR-155* and miR-155 correlated negatively with their respective targets during TLR7 stimulation. Purified PDCs were stimulated with 5 μg/mL R837 over a 24-hour time course and harvested at the indicated time points. miR-155* (C) and miR-155 (D) were analyzed with the TaqMan MicroRNA Expression Assay and normalized to the RNU48 levels. IRAKM (C) and TAB2 (D) mRNA were detected by qRT-PCR and normalized to the RPL13a RNA levels. These graphs show the fold induction calculated by normalizing the expression values at different time points to the 0-hour values. The data are representative of at least 3 independent experiments, each based on a different PDC preparation. (E) Immunoblot kinetics analysis for IRAKM and TAB2. An α-tubulin immunoblot is shown for equal loading control. Data are representative of 2 independent experiments.