ERG induces T-ALL in mice. (A) Life expectancy of ERG transgenic mice. Kaplan-Meier survival curves of ERG transgenic mice with T-ALL and wild-type littermate controls (WT). (B) An ERG transgenic mouse with T-ALL dissected to show its internal organs. There is enlargement of the thymus (T), spleen (S), and liver (L). (C) Histology of the thymus of an ERG transgenic mouse with T-ALL. An H&E-stained thymus showing dense leukemic cell infiltration. (D) Flow cytometric analysis of bone marrow from wild-type mice. (E) Flow cytometric analysis of bone marrow and thymus from transgenic ERG mice. The cell surface markers were measured relative to isotype control. The percentage of positive cells is indicated in the corner of each quadrant. (F) T-cell receptorβ rearrangements. PCR products showing Dβ2-Jβ rearrangements in thymus from 4 ERG transgenic mice compared with wild-type thymus and tail (germline). (G) Notch1 mutations. Insertion or deletion mutations in the Notch1 PEST domain in 5 ERG transgenic mice.