Strategy of FACS of different lymphocyte populations and purity of sorted populations. (A-G) FACS of a patient with T-cell LGL lymphocytosis (patient no. 2; Table 1) and (H-J) of a patient with NK-cell lymphocytosis (patient no. 6; Table 1). To sort lymphocyte subpopulations, PB MNCs were stained with antibodies against the following antigens: CD45, CD3, CD8, CD16/CD56, TCR αβ, and TCR γδ. Gates were first set on live and CD45⁺ cells (gray). Then gating of NK- and T-cell populations were done from CD16/CD56 versus CD3 scatter (A,H). CD3⁺ T cells were further sorted into CD8^neg and CD8⁺ populations (B) and into TCRαβ⁺ and TCRγδ⁺ T cells (E). Purity of respective sorted populations is presented (C,D,F,G,I,J).