In vivo therapeutic effects of TH-302 on 5T33MM model. 5T33MMvv mice were treated prophylactically with TH-302 for 3 weeks from day 1. (A) Serum paraprotein level (as determined by serum electrophoresis) was decreased after treatment with TH-302. (B) Histomorphometric analysis of MVD. MVD was determined by CD31 staining as described in “Methods.” In the area with the highest blood vessel density (hot spot), the number of blood vessels was counted per 0.22 mm2. (C) Hematoxylin and eosin staining of bone marrow section. Nuclei from apoptotic cells show condensed, fragmented morphology. Original magnification, × 63; n = 10/group. Detailed image information available in supplemental Methods. Quantitative data are the frequency of apoptotic MM cells in bone marrow sections. *P < .05, **P < .01, significantly different from vehicle. n = 10/group.
