Naive, memory, and effector CD4⁺ T-cell reconstitution after Ab-mediated CD4⁺ lymphocyte depletion. PB- and LN-derived mononuclear cells were obtained from healthy SIV-uninfected SMs and RMs. (A) Gating strategies used to identify naive (red line), effector (blue line), and memory (black line) CD4⁺ T cells are shown in a representative animal. (B-D) Fractions (B, D) and absolute numbers (C) of naive, effector and memory cells in the CD4⁺ T-cell pool were longitudinally assessed after antibody-mediated depletion of CD4⁺ lymphocytes in the PB (B-C) or LN (D) of SMs (left graphs) and RMs (right graphs). Values in panels B through D are means ± SD, and the dotted lines indicate the timing of anti-CD4 monoclonal antibody administrations.