The combination of PTL and TEM increases the overall oxidative insult to AML cells. Cells were treated with PTL, TEM, or PTL + TEM at the concentrations indicated alongside untreated controls. (A) Histogram overlay indicating relative oxidative changes in AML1 as measured by H₂DCF-DA (left column) and mBBr (right column). Untreated controls are indicated by the shaded blue histogram. Drug treatments are indicated by colored open histograms top row: untreated vs PTL, Middle row: untreated vs TEM, Bottom row: untreated vs PTL + TEM (B) Bar chart indicates the base-2 log fold change of mean fluorescence intensity measured by H₂DCF-DA (top left panel) or mBBr (top right panel). *P < .05 (n = 3; AML1).