Leukocyte adhesion (mean ± SEM) in trauma-stimulated cremaster muscle venules. Leukocytes adhesion (number of adherent cells/mm²) was investigated in surgically exteriorized cremaster muscle venules of WT control mice (n = 9), RAGE^−/− mice (n = 10), Icam1^−/− mice (n = 8), and RAGE^−/−Icam1^−/− mice (n = 3; A). To investigate the contribution of Mac-1 and LFA-1, leukocyte adhesion was also assessed in RAGE^−/− mice pretreated with Mac-1–blocking mAb Tib128 or LFA-1–blocking mAb Tib217 (n = 7), Icam1^−/− mice pretreated with Mac-1–blocking mAb Tib128 or LFA-1–blocking mAb Tib217 (n = 7), and WT mice pretreated with Mac-1–blocking mAb Tib128 and LFA-1–blocking mAb Tib217 (n = 3). (B) To investigate the role of endothelium-expressed RAGE, leukocyte adhesion was assessed in cremaster muscle venules of bone marrow chimeric mice lacking leukocyte expressed RAGE and ICAM-1 (RAGE^−/−Icam1^−/− into WT, n = 3) or endothelium-expressed RAGE and ICAM-1 (WT into RAGE^−/−Icam1^−/−, n = 3; C). WT bars (first bar on the left) are identical in panels A-C. In addition, LTB4-induced leukocyte adhesion was observed in cremaster muscle venules of WT mice (n = 16), RAGE^−/− mice (n = 10), and Icam1^−/− mice (n = 5) after 3 minutes of superfusion of the cremaster muscle with LTB4 shortly after exteriorization. Values are given as increase in leukocyte adhesion over baseline adhesion values (D). *Significant differences (P < .05) in leukocyte adhesion to WT control mice.