Leukocyte adhesion and spreading on sRAGE in vitro. Adhesion and spreading of isolated WT (black bar) and RAGE^−/− (gray bar) neutrophils on immobilized sRAGE or fibrinogen on stimulation with TNF-α (100 ng/mL), Mn²⁺ (1mM), or mock treatment (unstimulated), respectively, at 37°C for 30 minutes. Adherent WT and RAGE^−/− neutrophils in percentage of total cells added (n = 7, mean ± SD,; A), microscopic images of spreading on sRAGE (B), and cell area in square micrometers of adherent WT and RAGE^−/− neutrophils on immobilized sRAGE and fibrinogen without or with stimulation (n = 500, mean ± SEM; C). *Significant differences (P < .05) in neutrophil adhesion and spreading versus unstimulated WT. Differences between WT and RAGE^−/− cells were not significant. Bar in panel B represents 10 μm.