Cell culture and in vivo transplantation of muscle-derived Gr1^dimCD11b⁺ and Gr1⁻CD11b⁻ cells. (A) Images of cultured Gr1^dimCD11b⁺ cells 4 days after in Matrigel. (B-C) Immunofluorescence of cultured Gr1^dimCD11b⁺ cells with anti–rabbit GFAP antibody (red) and isolectin (green) 4 days after in Matrigel. (D) Immunofluorescence of cultured Gr1^dimCD11b⁺ cells with anti–rabbit von Willebrand Factor (VWF) antibody (arrow; red) and isolectin (green) 4 days after in Matrigel. (E) No isolectin and VWF expressions were observed in cultured Gr1⁻CD11b⁻ cells. (F) Red fluorescent signals indicate localization of transplanted C57BL/6 derived Gr1^dimCD11b⁺ cells (CD45.2). Ten days after cell injection, CD45.2 positive cells were present in the vascular wall of the vessels of Boy/J mice. (G) Growth of Gr1^dimCD11b⁺ cells in Matrigel culture. Total cells and isolectin/VWF double positive cells were counted at the indicated times. Quantitative analysis of hind-limb perfusion; (H) LDP index was significantly higher in the Gr1^dimCD11b⁺ cells (5 × 10⁵ cells /mouse) injected group compared with the control group injected with Gr1⁻ CD11b⁻ cells (5 × 10⁵ cells /mouse) 3 weeks after cell injection. Data are mean ± SEM; P = .003.