SATB1 expression is down-regulated by knockdown of STAT6 but not by knockdown of GATA3. (A) Cord blood and buffy coat CD4⁺ T cells were nucleofected with STAT6-siRNA or scrambled control siRNA, and cells were cultured in Th2-polarizing conditions. Cells from 3 to 7 independent experiments were harvested at indicated time points and analyzed using quantitative RT-PCR. The normalized expression (dCt) of STAT6 (left panel) and SATB1 (right panel) mRNA of STAT6-siRNA (solid line) and control siRNA (dashed line) nucleofected cells is presented. (B) Effect of STAT6 knockdown on SATB1 expression at 3-day time point in Th2 condition analyzed with Western blotting. Sc indicates scrambled control siRNA; and S6, STAT6-siRNA. Representative value of 3 independent experiments. Vertical lines have been inserted to indicate a repositioned gel lane. (C) Cord blood CD4⁺ T cells were nucleofected with GATA3-siRNA or scrambled control siRNA, and cells were cultured in Th2-polarizing conditions. Cells from 3 independent experiments were harvested at indicated time points and analyzed using quantitative RT-PCR. The normalized expression (dCt) of GATA3 (left panel) and SATB1 (right panel) mRNA of GATA3-siRNA (solid line) and control siRNA (dashed line) nucleofected cells is presented. (D) Effect of GATA3 knockdown on SATB1 expression at 1-day time point in Th2 condition analyzed with Western blotting. Sc indicates scrambled control siRNA; and G3, GATA3-siRNA. Representative value of 3 independent experiments. Vertical lines have been inserted to indicate a repositioned gel lane. *P < .05. **P < .01. ***P < .005.