Induction of IL-5 in SATB1-silenced Th2-polarizing cells is GATA3-dependent. Cord blood CD4⁺ T cells were nucleofected with siRNA oligonucleotides targeting SATB1 or GATA3 (1.5 μg specific siRNA and 1.5 μg scrambled control siRNA) or both (1.5 μg each siRNA) or only scrambled control siRNA (3 μg) and cells were cultured in Th2-polarizing conditions. (A-C) Samples of the 24-hour time point from 6 independent experiments were analyzed using quantitative RT-PCR. The normalized expression (dCt) of SATB1 (A), GATA3 (B), and IL-5 (C) mRNA of specific siRNA-treated and scrambled control siRNA nucleofected cells is presented. (D) The culture media of the 48-hour time point was measured for secreted IL-5 using Bio-Plex assay and is expressed in picograms per milliliter. Data are mean ± SD of 3 independent experiments. *P < .05. **P < .01. ***P < .005.