KLF4 induces cell death in cHL cell lines. (A) Expression of KLF4-ER fusion protein in KMH2-KLF4-ER and L428-KLF4-ER as well as in KM-H2-pcDNA and L428-pcDNA clones was investigated as described for Figure 2 legend. (B) KLF4 activation suppresses growth of cHL cell lines. The experiments were done as described for Figure 2B legend, except that cells were seeded at density 1 × 105/well because of the relatively slow growth kinetic of cHL cell lines. (C-D) Cell-cycle distribution was measured as indicated for Figure 2C and D. (E) KLF4 induces apoptosis in cHL cell lines (Figure 2E). (F) Caspase inhibition does not completely protect cHL cell lines from KLF4-induced apoptosis. A total of 2 × 105 cells/well of 6-well plate were seeded in 3 mL of the medium. Z-VAD.fmk was added at the day of seeding at concentration 320μM followed by 200nM of 4-OHT 2 hours later. Cell death is given as percentage of dead cells. Data are mean ± SD of 3 independent experiments.
