Proliferation of mononuclear cells is not affected in mice injected with M8-HLA-G1 cells. (A) Balb/c mice were injected subcutaneously with M8-pcDNA cells (left panel) or M8-HLA-G1 cells (right panel). At the indicated time points, mononuclear cells were isolated from lymph nodes (LN), spleens, or peripheral blood (PBMC), and their proliferative capacity was tested in response to M8-pcDNA or M8-HLA-G1 cells at a responder:stimulator ratio of 1:1. Mononuclear cells cultured in medium alone were used as a control. Results represent the means ± SD of triplicates from 1 representative experiment of 3. The numbers indicated between parentheses are the P values. (B) Experiments were conducted as described in panel A except that M8-HLA-G1 cells in the presence of the anti–HLA-G 87G mAb were used. Left panel shows mice injected with M8-pcDNA cells; right panel, mice injected with M8-HLA-G1 cells. Results represent the means ± SD of triplicates from 1 representative experiment of 3. *P < .05; **P < .001; ***P < .005.