EGR-1–induced expression in endothelial cell lines after contact with T lymphoma cells. (A) After in vitro contact with freshly isolated murine thymocytes, 164T2, or 164T2S11 T lymphoma cell lines, bEnd.3 cells were harvested and total RNA was extracted and then subjected to reverse-transcribed–polymerase chain reaction (RT-PCR) to quantify EGR-1 expression. (B) Expression of L-selectin in bEnd.3 cells after contact with 164T2 T lymphoma cells as a control of bEnd.3 purity after sorting. No expression of L-selectin was detected in bEnd.3 or after purification after coculture with T lymphoma cells. The 164T2 cells were used as a positive control for L-selectin expression. In both panels A and B, the control included bEnd.3 cells incubated without lymphoid cells in the same culture conditions and GAPDH expression was used as internal control. Bars represent SD; *P ≤ .05.