Figure 1
![Figure 1. Expression of PAI-1 in catecholaminergic cells. Left Panel: Northern blot analysis of PAI-1 mRNA expression in rat PC12 pheochromocytoma cells. Twenty-five μg of total RNA from either untreated or NGF-treated PC12 cells were loaded and separated on a 1% formaldehyde agarose gel. [α-32P]-labeled PAI-1 cDNA and rat cyclophilin cDNA were used as specific and control probes, respectively. The positions of 28 S and 18 S ribosomal RNA are shown. Right Panel: Expression of PAI-1 antigen in PC12 cells (A) and bovine adrenal medulla (B). Expression of PAI-1 antigen was investigated by Western blot analyses using specific anti–PAI-1 antibodies, or control normal rabbit IgG.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/117/26/10.1182_blood-2010-05-287672/5/zh89991174100001.jpeg?Expires=1735821888&Signature=lsiWkRCz42kX~jE-klErZ8h7W9kQCrjcHAVMb70YHeCpzk9aiL-g92fdtFBKa6tdjv0gXD9doX3zlumFvKSA3-fD2hcomDFmMUz48tIDguVBwv8S9fyL9Nq2oIiMhQOBcT6U15OcSbmQjCmRJiypCBg4Qf606k6fw8~1L57yIzRL2dbCtTkrfcyf6bqYDoBZR-bz2bJdg3AmSGsiVh~c6sCgcC2d5xbANcr7DGh-3kwS3Kz9tA60Q1-DgWsraJuOPaKE5TrRgFKvNp~-u5xfwdyUNweHoh9SvzQXdx2ispExjJafhXaq32~UpUcaXgEPV~nIzFZ63WCe0OtBQ3f84Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression of PAI-1 in catecholaminergic cells. Left Panel: Northern blot analysis of PAI-1 mRNA expression in rat PC12 pheochromocytoma cells. Twenty-five μg of total RNA from either untreated or NGF-treated PC12 cells were loaded and separated on a 1% formaldehyde agarose gel. [α-32P]-labeled PAI-1 cDNA and rat cyclophilin cDNA were used as specific and control probes, respectively. The positions of 28 S and 18 S ribosomal RNA are shown. Right Panel: Expression of PAI-1 antigen in PC12 cells (A) and bovine adrenal medulla (B). Expression of PAI-1 antigen was investigated by Western blot analyses using specific anti–PAI-1 antibodies, or control normal rabbit IgG.
