Figure 2
![Figure 2. Colocalization of PAI-1 and norepinephrine in sucrose density gradient fractions. PC12 cells were labeled with [3H]norepinephrine and fractionated on a continuous sucrose density gradient as described in “Sucrose gradient fractionation.” Fractions were collected and assayed for [3H]norepinephrine by liquid scintillation counting (squares), PAI-1 by ELISA (circles), and sucrose concentration by refractometry (triangles; A). Fractions 10 to 19 were further analyzed by Western blotting with anti–PAI-1 antibody as described in “Western blotting” (B).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/117/26/10.1182_blood-2010-05-287672/5/zh89991174100002.jpeg?Expires=1735821875&Signature=4JQ94TAS5xPmgkGEqFRoUD3LGFRtZC1E7I4gJcYbf5pZCyvwCcqJm49btt5Xp0-jEYw08mJvKutiDcS4qCUgshmT3InqiEZFSRJjt70bIE9iGS7aBfcj0mm5kB743RBKbgYMDPrHizKzN0O4At0ACMVlDNZ2rlBjgcHfIsk6flF4zvCg23mzba4Q5ksqPUK83Hte~KFuzx0u9TnUg56u8GzwZOCxxNMx50jL369ndR5JbMeV1vpL~1Lpb0Q0Yv6Um--jR-iWyW9GrjKa3NYNFFQ2XR3CSxh7ooq2-vlPrBiVAYXgxFME45ZLtlVWdwST-eoSQ1BzxJz7RTUYgcWg9A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Colocalization of PAI-1 and norepinephrine in sucrose density gradient fractions. PC12 cells were labeled with [3H]norepinephrine and fractionated on a continuous sucrose density gradient as described in “Sucrose gradient fractionation.” Fractions were collected and assayed for [3H]norepinephrine by liquid scintillation counting (squares), PAI-1 by ELISA (circles), and sucrose concentration by refractometry (triangles; A). Fractions 10 to 19 were further analyzed by Western blotting with anti–PAI-1 antibody as described in “Western blotting” (B).
