Figure 2
Untagged p40phox fully rescues NADPH oxidase activity in p40phox−/− neutrophils. A quantity of 1 × 106 wt BMNs (▾) or p40phox−/− BMNs expressing GFP (○) or untagged p40phox (p40-IRES-GFP; 
) were pre-incubated with luminol/HRP before addition to fMLP (A) or PMA (C) or pre-incubated with luminol before addition to serum-S aureus (B), as described in the Methods. Shown are total integrated ROS responses for 2 independent experiments (mean ± range), expressed as a percentage of the response in wt BMNs, as well as profiles of kinetics of ROS production, measured in relative light units per second (RLU/s) (mean ± range), from 1 representative experiment performed in duplicate.
