Figure 6
Phosphorylation of p40phox on T154 is unaffected in p40phox-R58A, -S315A, or -ΔSH3 neutrophils. p40phox−/− (−/−), wt (+/+), p40phox+/− (+/−), p40phoxR58A/R58A (R58A/R58A) BMNs, or p40phox−/− BMNs reconstituted with mutated versions of p40phox as indicated were incubated without or with S aureus (ratio S aureus/BMN of 20:1) for 20 minutes at 37°C. Cell lysates were subjected to SDS-PAGE, after which immunoblotting was performed using an antibody directed against p40phox phosphorylated on T154 or total p40phox as a loading control. Representative blots from 2-5 independent experiments are shown.
