Identification of STAT5 target genes in HSCs and progenitors. Cord blood cells were transduced with MiNR1 (control) and STAT5-wt ER retroviral vectors and sorted into HSC, CMP, GMP, and MEP fractions. Cells were stimulated with 100nM 4-OHT for 24 hours, and then RNA was extracted and used for Illumina BeadChip array analysis. Significantly expressed STAT5 target genes in HSC and progenitor compartments (> 2-fold change in gene expression) are shown. (B) Venn diagram showing STAT5 target genes in HSC, GMP, and MEP fractions. (C) Verification of STAT5 target genes by real-time Q-PCR. (D) Schematic representation of the identification of GATA1-independent STAT5 target genes. Cord blood CD34⁺ cells were transduced with MiNR1 or STAT5A-ER and luciferase RNAi or GATA1 RNAi vectors, as described previously.²⁴  Cells were stimulated with 100nM 4-OHT for 24 hours, and then RNA was extracted and used for Illumina microarray analysis. Thus, significantly expressed STAT5 target genes could be identified in the absence or presence of GATA1 (> 2-fold change in gene expression). (E) Venn diagram showing STAT5 target genes in HSC and GATA1-independent target genes. (F) dataset from the Illumina BeadChip arrays showing expression of STAT5 target genes. Perfect palindromic STAT5-binding sites defined as TTC(n₃)GAA are highlighted in green.