Glucose uptake is enhanced upon STAT5 activation. (A) Cord blood CD34⁺ cells were transduced with STAT5-ER and cells were stimulated with 100nM 4-OH for 24 hours as indicated. The glucose probe 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG) was added for 3 hours, and then glucose uptake was analyzed by FACS in the CD34⁺/CD38⁻ HSC compartment and the CD34⁺/CD38⁺ multipotent progenitor (MPP) compartment. (B) Cord blood CD34⁺ cells were transduced with MiNR1 control or STAT5-ER together with luciferase RNAi control or HIF2α RNAi constructs, and double-transduced cells were sorted. Sorted cells were stimulated with 100nM 4-OH for 24 hours, and then RNA was isolated for real-time Q-PCR analysis. *P < .05.