Cx43 deficiency in OB/P compartments does not modify steady-state hematopoiesis but impair radioprotection in primary recipient mice. (A) BM cellularity (2 femora, 2 tibiae, and pelvis) in WT and OB/P Cx43-deficient mice. (B) BM content of HSC/P subpopulations in WT and OB/P Cx43-deficient BM. LT indicates long-term; ST, short-term; MPP, multipotent progenitor; CMP, common myeloid progenitor; GMP, glanulocyte/macrophage progenitors; MEP, megakarycyte/erythroid progenitor; and CLP, common lymphoid progenitors. (C) Hematopoietic progenitor content (CFU-granulocyte-macrophage [GM], erythroid burst-forming units [BFU-E], and CFU-Mix) in the BM of WT and OB/P Cx43-deficient mice. (D) Survival curve of lethally irradiated WT (dashed line) or OB/P Cx43-deficient (solid line) recipient mice transplanted with 1 × 10⁴ WT BM cells (low dose). (E) Percentage of chimerism in WT (empty circles) and OB/P Cx43 deficient mice (solid circles) transplanted with 3 × 10⁵ WT CD45.1⁺ BM cells. Data are shown as mean ± SEM, of 2 independent experiments, with a minimum of 7 mice per group.